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KMID : 0985420120340020093
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Laboratory Medicine and Quality Assurance
2012 Volume.34 No. 2 p.93 ~ p.97
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Comparison of Rapid Antigen Test and Real-Time Reverse Transcription PCR for the Detection of Influenza B Virus
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Park Min-Jeong
Kim Han-Sung
Lee Young-Kyung
Kang Hee-Jung
Kim Jae-Seok
Song Won-Keun
Lee Kyu-Man
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Abstract
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Background: Rapid antigen test and real-time reverse transcription PCR (rRT-PCR) are widely used for the detection of influenza A virus. In this study, we evaluated and compared the effectiveness of a rapid antigen test, currently used for detecting influenza B virus, with the effectiveness of using rRT-PCR for the same purpose. Methods: Samples obtained from 92 patients during an outbreak of influenza B were assessed using the rapid antigen test (SD BIOLINE Influenza Ag; SD, Korea) and rRT-PCR (Anyplex FluA/B Real-time Detection; Seegene, Korea). Results: The sensitivity and specificity of the rapid antigen test were 69% and 100%, respectively, in detecting influenza B when compared to rRT-PCR. Twenty-nine patients (31.5%) were positive for both rapid antigen test and rRT-PCR, while 50 (54.3%) were negative for both rapid antigen test and rRT-PCR. The overall concordance rate between rapid antigen test and rRT-PCR was 85.9%. Thirteen
patients (14.1%) were positive only for rRT-PCR. Conclusions: The rapid antigen test showed high specificity and good correlation with rRT-PCR and is likely to be as useful in the detection of influenza B viruses.
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KEYWORD
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Influenza B virus, Rapid antigen test, Real-time PCR
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